Insertion junction: LMJ.RY0402.069785_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.069785
Chromosome:chromosome 3
Location:3287114
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g166000 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGAGCAGGTGTACAAAAAAACAACCAA
Internal bar code:CTTAAAAATAGTTGACGGTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:132
LEAP-Seq percent confirming:80.7099
LEAP-Seq n confirming:1728
LEAP-Seq n nonconfirming:413
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGGCTAGGACTCACTG
Suggested primer 2:AGTTGTCAGGGGTTTGATGC