| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069813 |
| Chromosome: | chromosome 3 |
| Location: | 383322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144867 | (1 of 1) K18624 - macrophage erythroblast attacher (MAEA, EMP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCAGCAAGGCCAGCCCGATAGTCAAGA |
| Internal bar code: | CCGCCTGGCTGAGTTCGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 64.4383 |
| LEAP-Seq n confirming: | 694 |
| LEAP-Seq n nonconfirming: | 383 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGATGAGTGTGGGGTAT |
| Suggested primer 2: | CCCATGAACGGCTACAACTT |