Insertion junction: LMJ.RY0402.069822_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.069822
Chromosome:chromosome 17
Location:1513274
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACAAGGACATCGCAGGCAGCCTGCGCCT
Internal bar code:TGGCGTCAGTCAGCGCGAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:982
LEAP-Seq percent confirming:96.8111
LEAP-Seq n confirming:4402
LEAP-Seq n nonconfirming:145
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGAGGGTAAGAGGGAGAG
Suggested primer 2:CATGAATGAGCGATGAATGG