Insertion junction: LMJ.RY0402.069823_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.069823
Chromosome:chromosome 9
Location:703403
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g402750 COG2 Component of oligomeric golgi complex; (1 of 1) PTHR12961//PTHR12961:SF0 - CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2 // CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCTTCCTGGAGCTGCTGCCGCTGCCA
Internal bar code:GGGGCCTTCCTAGGTTGAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:21
LEAP-Seq percent confirming:97.1591
LEAP-Seq n confirming:342
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGTGTAGGTGATGAGCA
Suggested primer 2:TTGCTCTGCTGCTCTGTTGT