| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069831 |
| Chromosome: | chromosome 2 |
| Location: | 4724369 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102050 | FAP328,CSE15 | Flagellar Associated Protein 328 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGGCGGTGTGTACCCCTTTCCGTCAGT |
| Internal bar code: | TAGCACACGCCGCTGCACATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTCTAATCACGCTCCGT |
| Suggested primer 2: | CAACGGTCTTCCAACAACCT |