| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.069924 |
| Chromosome: | chromosome 10 |
| Location: | 368849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420500 | (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACCTGCATGCTTGCCCACTGACCTCCC |
| Internal bar code: | CGATACTGGTTGGTGTGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 98.8327 |
| LEAP-Seq n confirming: | 1016 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACGACTACTCGGCACTG |
| Suggested primer 2: | TCGTACAGCGGGATGATGTA |