| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.069985 |
| Chromosome: | chromosome 8 |
| Location: | 4249382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380763 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACGTGTGATGCTGGCTGGGTGAGCGGC |
| Internal bar code: | CGGAACGCGAGCTCGTTGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 68.4508 |
| LEAP-Seq n confirming: | 10180 |
| LEAP-Seq n nonconfirming: | 4692 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTAACGCGATCCCACT |
| Suggested primer 2: | ACCCAGATTGCTTGACCTTG |