Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.069988 |
Chromosome: | chromosome_2 |
Location: | 3520391 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095126 | DPE2 | alpha-1,4-glucanotransferase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTTCATAGGTAGAGACTTCGTTTCGTGGT |
Internal bar code: | CGGTGACGTTACAGGTGGGAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 920 |
LEAP-Seq percent confirming: | 99.1398 |
LEAP-Seq n confirming: | 9451 |
LEAP-Seq n nonconfirming: | 82 |
LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGCGGACGGAAGTAAGTA |
Suggested primer 2: | TCCAAACCTGAAGCATTTCC |