| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.069990 |
| Chromosome: | chromosome 11 |
| Location: | 2631320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476400 | MAG1 | DNA-3-methyladenine glycosylase II; (1 of 1) 3.2.2.21 - DNA-3-methyladenine glycosylase II / DNA-3-methyladenine glycosidase II | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTCGGGCGTTCGCCTTCAAGCTTCGC |
| Internal bar code: | CCTGAAACGAAAACGTCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.7486 |
| LEAP-Seq n confirming: | 1587 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCCTGCTCTGCTGTG |
| Suggested primer 2: | CGTAACACCCCTCATCTCGT |