| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070004 |
| Chromosome: | chromosome 6 |
| Location: | 3526323 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278109 | FAD2B | Putative Oleate/fatty acid desaturase, _-12; (1 of 1) 1.14.15.3 - Alkane 1-monooxygenase / Omega-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGGCCTGCTGCGCCCCCCCTACCGTCT |
| Internal bar code: | CTAAGCGTCCGGCGTGGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 210 |
| LEAP-Seq percent confirming: | 95.7939 |
| LEAP-Seq n confirming: | 2938 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATGCCATAAACCAACCC |
| Suggested primer 2: | TCAACTGTGGAGGGGTTAGG |