| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070008 |
| Chromosome: | chromosome 2 |
| Location: | 4069378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097400 | EIF5A | (1 of 1) K03263 - translation initiation factor 5A (EIF5A); Eukaryotic initiation factor, eIF-5A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTAAAAGGCGCGGCCCGGTCTGACTAC |
| Internal bar code: | GCAAATTGACGACTCGTCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.8099 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCGCGTCCTAAATGCTC |
| Suggested primer 2: | TGACCTTTTGCCCACATACA |