| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070017 |
| Chromosome: | chromosome_9 |
| Location: | 4477400 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTGGATGGAGGTAGAGGCGGGGCAGCAAG |
| Internal bar code: | CGCGCCTCGATAGCCAGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 |
| LEAP-Seq percent confirming: | 50.3435 |
| LEAP-Seq n confirming: | 2125 |
| LEAP-Seq n nonconfirming: | 2096 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAAGTTGGAAGGGCTAC |
| Suggested primer 2: | CTCAACTGCTCACCCTCACA |