| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070052 |
| Chromosome: | chromosome 3 |
| Location: | 5843658 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189350 | (1 of 1) PTHR36352:SF1 - EXPRESSED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGACATCGGCATGGCGTTGGGTCTGGTA |
| Internal bar code: | TTGCTTTGCCGGCTCTCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTAGCACTAAGTGGCCTG |
| Suggested primer 2: | CATGGCTCTAGCTCCCACTC |