| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070080 |
| Chromosome: | chromosome 10 |
| Location: | 5039546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455700 | PAP5 | Non-canonical poly(A) polymerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGGATGCAGCGGAGAAGCGGCGGCAT |
| Internal bar code: | ACCAGCCTCGTTCAGCACGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.8369 |
| LEAP-Seq n confirming: | 612 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGCATTTGGGGCTACAT |
| Suggested primer 2: | ATCTGCATCTCCACCTCCAC |