| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070101 |
| Chromosome: | chromosome 12 |
| Location: | 7432705 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556600 | GAP4,GAPN1 | (1 of 1) 1.2.1.9 - Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) / Triosephosphate dehydrogenase; Glyceraldehyde 3-phosphate dehydrogenase, nonphosphorylating | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACACGCCAAATCCCAACCATGATTCCAA |
| Internal bar code: | CACCTGAACAGTTCGCAGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 96.3107 |
| LEAP-Seq n confirming: | 3707 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGGAGGGAGGGAGGAAG |
| Suggested primer 2: | CCTGTCGTGGTGTTGTGAAC |