| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070146 |
| Chromosome: | chromosome 3 |
| Location: | 8178997 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200250 | ICL3 | Putative isocitrate lyase/malate synthase; (1 of 1) 2.7.8.23 - Carboxyvinyl-carboxyphosphonate phosphorylmutase / CPEP phosphonomutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGCAACAGCCAGCGTCAGTGGGGCATG |
| Internal bar code: | CGTCCCAACTCCGATGGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.8765 |
| LEAP-Seq n confirming: | 4044 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGTTGTTTGAGGGAGC |
| Suggested primer 2: | GTTGACGTTGAACCAATCCC |