| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070154 |
| Chromosome: | chromosome 5 |
| Location: | 1640315 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACGCACCTGTATGGCCCCCGACCAGCT |
| Internal bar code: | GTTTATTTTATCGGCCATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 45.8422 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 254 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACTTGGGGAGGATTGGG |
| Suggested primer 2: | GAGGTGCGTGTATGAGGGTT |