Insertion junction: LMJ.RY0402.070173_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.070173
Chromosome:chromosome 1
Location:1552377
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g008100 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCACCTTCTGTCCCGAACGGGCGCAGTT
Internal bar code:CCACCAGCAACTGATAGACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:276
LEAP-Seq percent confirming:37.2178
LEAP-Seq n confirming:610
LEAP-Seq n nonconfirming:1029
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGGGTGCAAAACATTG
Suggested primer 2:AATCCAAAAGAAAATGGGGG