| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070199 |
| Chromosome: | chromosome 8 |
| Location: | 9214 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGCGTGCCGCCCTCGCAGAACTCCAG |
| Internal bar code: | GTTGGTCGCATAATCCTTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.2727 |
| LEAP-Seq n confirming: | 5050 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACTGACCAGTGTGGTGG |
| Suggested primer 2: | GCCGGAACTTCGTCAAATTA |