| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070207 |
| Chromosome: | chromosome 7 |
| Location: | 4224001 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342000 | (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGAGAGCAGGAGGGGTGGGGGCGGGGG |
| Internal bar code: | GCTCCACGTCTTCGCGTTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAATTGCGAGTTGAAAA |
| Suggested primer 2: | AGACGACTACCAGGTGGGTG |