| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070229 |
| Chromosome: | chromosome 12 |
| Location: | 8668549 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546600 | FEA2 | Fe-assimilating protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCCCGCGTTGACCTGATGCGCGAGCTG |
| Internal bar code: | ACTTTGGATGCGGAAGTGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 94.5736 |
| LEAP-Seq n confirming: | 2318 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACGCTCAGTAGCACAC |
| Suggested primer 2: | CACGTCAGTCCGCTATGCTA |