| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070236 |
| Chromosome: | chromosome 2 |
| Location: | 8393628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141586 | (1 of 13) IPR001487 - Bromodomain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGGCAGGTTTATGCCTACCTGATACC |
| Internal bar code: | GTCCCCAGAAAAGAATCTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 98.773 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCACGTGCTGTAGTCAA |
| Suggested primer 2: | ATGTGAGGGATGACTGAGGC |