Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.070270 |
Chromosome: | chromosome_3 |
Location: | 4792653 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178500 | RIB43,RIB43A | Flagellar protofilament ribbon protein | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACATGGCATACCGTCCTCGAATTGAGAGCC |
Internal bar code: | AGTCTGGCTCTGGCCTGGTTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 95 |
LEAP-Seq percent confirming: | 99.4483 |
LEAP-Seq n confirming: | 721 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTATCGGTTGAGCGTTGGT |
Suggested primer 2: | ACACCAAGGTGAAGGGTCAG |