| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070279 |
| Chromosome: | chromosome 2 |
| Location: | 3833578 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095650 | (1 of 5) 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase / HNA-3-monooxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTCCTGCATTGCACAATCCCAACCGC |
| Internal bar code: | GGGGGATCACGGACGGATTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 74.0619 |
| LEAP-Seq n confirming: | 1579 |
| LEAP-Seq n nonconfirming: | 553 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGAGTATCGTCACAGCA |
| Suggested primer 2: | AGGGGACGAGTCATTACGTG |