| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070281 |
| Chromosome: | chromosome 13 |
| Location: | 1147349 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569550 | (1 of 17) PF08016 - Polycystin cation channel (PKD_channel) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGCTGTCTGCGTTCCCAATTTCCGCTG |
| Internal bar code: | GGGGCTTTTTTCACGTGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 98.3966 |
| LEAP-Seq n confirming: | 1841 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGCAGTATTGGCAGCA |
| Suggested primer 2: | CAAGTGTTCCCCGCATAACT |