Insertion junction: LMJ.RY0402.070296_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.070296
Chromosome:chromosome_5
Location:1182035
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre05.g245500 FAP175,ANK24 Flagellar Associated Protein with ankyrin repeats sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGCATGTAGCTCGCGATGTCAAGGTGCACG
Internal bar code:ATCCGCCTGCAGAGGGAACCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:5.37728
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:1091
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACTGTCTCATGCCGTA
Suggested primer 2:CCAGGTACAGGTCGAGGGTA