| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070303 |
| Chromosome: | chromosome 10 |
| Location: | 3016858 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441050 | (1 of 2) PTHR12321:SF55 - PHD FINGER PROTEIN ALFIN-LIKE 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCACACAGCCGTGTTGCCCTATGTGAC |
| Internal bar code: | TTTTGGACTGACGGCGGATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 98.8026 |
| LEAP-Seq n confirming: | 3053 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCCTTCTTGCTACGC |
| Suggested primer 2: | CTGCAACAAACTGACAGGGA |