Insertion junction: LMJ.RY0402.070305_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.070305
Chromosome:chromosome 8
Location:3351587
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g374200 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACCTGACGAAGAATCGCACAACACACA
Internal bar code:GCGACGCTGAGCCCCAATAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:32
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGGTTATTGGTGGTGAT
Suggested primer 2:CAACGAATTCCATTCCGAGT