| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070309 |
| Chromosome: | chromosome 9 |
| Location: | 4076968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393432 | (1 of 1) IPR002421//IPR029060 - 5'-3' exonuclease, N-terminal // PIN domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCTACACGCAGCAGCACTTGACATG |
| Internal bar code: | CGGCGATCGTAAAAAGCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 97.2594 |
| LEAP-Seq n confirming: | 4791 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGTAGCTGACGATGGC |
| Suggested primer 2: | ACACGACCGCTATTTCCAAC |