| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070312 |
| Chromosome: | chromosome 16 |
| Location: | 4374445 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671450 | FAO12 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF150 - FAD NAD BINDING OXIDOREDUCTASES // OXIDOREDUCTASE TDA3-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGCACAGTGCATGCGCGTATGTATGT |
| Internal bar code: | GCTTTGGCACAACCTTTCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 99.6942 |
| LEAP-Seq n confirming: | 652 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCTCAGGGCAGATAGGT |
| Suggested primer 2: | GTTCAAACTAGCTGCTCCCG |