| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070313 |
| Chromosome: | chromosome 2 |
| Location: | 1935724 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCGTGGCAGCACCAGCAATAGCACCTC |
| Internal bar code: | CCGTCGCGTAGGGCGTATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 80.4768 |
| LEAP-Seq n confirming: | 12152 |
| LEAP-Seq n nonconfirming: | 2948 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTTCCACTCTTGGCGT |
| Suggested primer 2: | GGAAGAGGTCATCACACGGT |