| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070332 |
| Chromosome: | chromosome 5 |
| Location: | 358713 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233305 | (1 of 1) K07071 - uncharacterized protein (K07071) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAACCACGTTCGCCGCGCCGCGACGAG |
| Internal bar code: | GACACGCAGACTTAATAGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 98.8604 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACTTCCAATCTGCTCAC |
| Suggested primer 2: | GTTGACACGTCACCCAGATG |