| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070365 |
| Chromosome: | chromosome 8 |
| Location: | 3390471 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374400 | (1 of 1) PTHR19920//PTHR19920:SF0 - WD40 PROTEIN CIAO1 // CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACGACGCGCCCCGCTGACACGCCGC |
| Internal bar code: | TAGGGGAGGTTTGTGGAGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 93.9468 |
| LEAP-Seq n confirming: | 2297 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCGGTGTCCAATACTGT |
| Suggested primer 2: | GAGAAGGGACAGGCTCACAG |