Insertion junction: LMJ.RY0402.070391_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.070391
Chromosome:chromosome 7
Location:1234124
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321350 (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCACCAGCCCCACCCATCTCACACACC
Internal bar code:GATGGCGGCTCAGGCTGTCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:567
LEAP-Seq percent confirming:74.4249
LEAP-Seq n confirming:1100
LEAP-Seq n nonconfirming:378
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACGAAATCACGCTACCCAC
Suggested primer 2:ATGTGGCGACCAGAACTACC