Insertion junction: LMJ.RY0402.070397_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.070397
Chromosome:chromosome 13
Location:867404
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g567450 HON1 Histone H1; (1 of 3) K11275 - histone H1/5 (H1_5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAAAGTCAGGTGAAGCTTGAATAGCTGT
Internal bar code:CGGCTCAGCGAACCCAGCTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:946
LEAP-Seq percent confirming:98.8929
LEAP-Seq n confirming:4109
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGGTTGATTTGCACAC
Suggested primer 2:CACCTTCCCCACACAAGTCT