Insertion junction: LMJ.RY0402.070424_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.070424
Chromosome:chromosome 17
Location:6148364
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g741601 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACACAACCAAAGCCCCTGCCTGCCGAC
Internal bar code:CGTGCCACGCTCTCACGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:92.387
LEAP-Seq n confirming:1165
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTGAACCCTTACTTC
Suggested primer 2:AGGTGTGTCAACACGATGGA