| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070433 |
| Chromosome: | chromosome_16 |
| Location: | 3173374 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g666150 | DCC2,ODA1,DC2 | Flagellar outer dynein arm-docking complex protein 2 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAGCAACCTGGAGCGGGAGCTGGCGAAGC |
| Internal bar code: | GGCGTACCCCACCTCGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 93.3198 |
| LEAP-Seq n confirming: | 922 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAACGAGCAGCTCAAGA |
| Suggested primer 2: | CTGCTGAGGACAGAGTTCCC |