Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.070433 |
Chromosome: | chromosome_16 |
Location: | 3173397 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g666150 | DCC2,ODA1,DC2 | Flagellar outer dynein arm-docking complex protein 2 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCCGCCGGAGGTTATTTATAGACTCCCGC |
Internal bar code: | CCACGAGCACAATGCCCTCTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 870 |
LEAP-Seq percent confirming: | 99.4059 |
LEAP-Seq n confirming: | 1506 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAGAACGAGCAGCTCAAGA |
Suggested primer 2: | CTGCTGAGGACAGAGTTCCC |