| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070438 |
| Chromosome: | chromosome 7 |
| Location: | 229791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313700 | TSX2,GTS2,TSE2,GTS2:GLTX | Glutamyl/glutaminyl-tRNA synthetase; (1 of 2) 6.1.1.17 - Glutamate--tRNA ligase / Glutamyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCTGGACCCTGCCACAACCCCGCCCT |
| Internal bar code: | ACTCGGCTCGGCGCTGCGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 99.2982 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGTCCGCATAACGAGG |
| Suggested primer 2: | CTCTTCTCCCTAACCGGTCC |