| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070467 |
| Chromosome: | chromosome 12 |
| Location: | 3852940 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515450 | UBC22 | (1 of 2) K10573 - ubiquitin-conjugating enzyme E2 A (UBE2A, UBC2, RAD6A); E2 Ubiquitin conjugating enzyme | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTCTTTTTCCCTTTTGGGCCCACGGG |
| Internal bar code: | ATACTTCTACTGCGTATACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 72.7844 |
| LEAP-Seq n confirming: | 1043 |
| LEAP-Seq n nonconfirming: | 390 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACCATCAGGTTGCTCT |
| Suggested primer 2: | ATCAAGAGTGAGTTGCCGCT |