| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070467 |
| Chromosome: | chromosome 17 |
| Location: | 3420948 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724300 | PSAK1,PSAK | Photosystem I reaction center subunit K; (1 of 1) K02698 - photosystem I subunit X (psaK) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGGGCAGTGAGAGATCCGACTTTGTC |
| Internal bar code: | CGTGCAATCTTCGCTGACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.6396 |
| LEAP-Seq n confirming: | 3871 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGATAGTGCTCGTCAC |
| Suggested primer 2: | ACAGCAAGTCACGTCGACAC |