| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070517 |
| Chromosome: | chromosome 12 |
| Location: | 7557480 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555550 | (1 of 1) K05768 - gelsolin (GSN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCCAGCCTGGTCAAGGCCGTCAAGGCG |
| Internal bar code: | GCGGGACGCCGCCGACGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 44 |
| LEAP-Seq percent confirming: | 1.68126 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2807 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACGCCGGTAATACACCT |
| Suggested primer 2: | GTCAGTTGCGACGTCAAAGA |