| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070523 |
| Chromosome: | chromosome 3 |
| Location: | 43914 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143747 | 3'UTR | ||
| Cre03.g143767 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTGCGATAGCTCTGATTGCATGTCAAC |
| Internal bar code: | GTGACGCCCAACAGATGCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 87 |
| LEAP-Seq percent confirming: | 59.6169 |
| LEAP-Seq n confirming: | 5291 |
| LEAP-Seq n nonconfirming: | 3584 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTGCACATTGGTCTTGT |
| Suggested primer 2: | GGCCTCCTGTATGCACATTT |