| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070540 |
| Chromosome: | chromosome 11 |
| Location: | 1621676 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467766 | (1 of 1) 6.5.1.3 - RNA ligase (ATP) / Ribonucleic ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATCGAAGGGGTACCTATCGCATTGCACT |
| Internal bar code: | GTCAGTCCGTCAATAGAGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 1672 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTTATTAAGGGGCAGGT |
| Suggested primer 2: | AGCATACCTGAAGGTGGGTG |