Insertion junction: LMJ.RY0402.070546_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.070546
Chromosome:chromosome 9
Location:3256091
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g387875 IPY3 Soluble inorganic pyrophosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGCCAATCCCTGCACCGCCGGGCATGG
Internal bar code:GTGTTCTCACTCCGGGGACAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:80.726
LEAP-Seq n confirming:6605
LEAP-Seq n nonconfirming:1577
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCAGGCATACAAGTTT
Suggested primer 2:CTGCTTCTTGACGAACACCA