| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070551 |
| Chromosome: | chromosome 12 |
| Location: | 3917975 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516050 | (1 of 1) PF00498//PF13920 - FHA domain (FHA) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCGCGGGGTGGCTGGCACAAAAGCAA |
| Internal bar code: | GCGAGGCCTGTAAGGTGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 98.373 |
| LEAP-Seq n confirming: | 1572 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGGCAGCCTTATTCTGA |
| Suggested primer 2: | GTACAGCAGGTGGCAAGTGA |