| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070552 |
| Chromosome: | chromosome 16 |
| Location: | 7693655 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690767 | (1 of 3) PF02737 - 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (3HCDH_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTAGCCGCTACCTAAATGCCTACTCGC |
| Internal bar code: | GGCCCTTTAAGAATCCCGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 99.8344 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCATCTCAGTTTGTGCC |
| Suggested primer 2: | GGAAGTTCCAGTTGCTCTGC |