Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.070558 |
Chromosome: | chromosome 6 |
Location: | 7668661 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g301600 | MAN2,EDEM1 | a-1%252C2-mannosidase II putative; (1 of 1) K10085 - ER degradation enhancer, mannosidase alpha-like 2 (EDEM2) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTTTTTCCTCACCTGAGCCGCTTCAC |
Internal bar code: | GCATATTTCGCCTTACAGTATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 295 |
LEAP-Seq percent confirming: | 92.9134 |
LEAP-Seq n confirming: | 118 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCATTCAGTTCTGTGAGCC |
Suggested primer 2: | ATGTACGCACGTAAGAGGGG |