| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070560 |
| Chromosome: | chromosome 2 |
| Location: | 6605912 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117300 | MRPL45 | (1 of 1) K17426 - large subunit ribosomal protein L45 (MRPL45); Putative TIM44 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTCGTTGCACCCTGCCCCCCCTGCGGT |
| Internal bar code: | AGGAACAGGTTACGGACTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 80.3319 |
| LEAP-Seq n confirming: | 968 |
| LEAP-Seq n nonconfirming: | 237 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTCCGGGTAGCAGACAC |
| Suggested primer 2: | GCAGTATGTGACGGAGCAAA |