| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070564 |
| Chromosome: | chromosome_6 |
| Location: | 1731022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g261750 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGCTGCATGCTACACCATGCTATCTGG |
| Internal bar code: | CGACGCCGGTGAACGGGCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 67.2358 |
| LEAP-Seq n confirming: | 1654 |
| LEAP-Seq n nonconfirming: | 806 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGAAGGTCACCGCCTGT |
| Suggested primer 2: | TGCATTTGCAAAACCCACTA |